--- title: FAQs description: got questions about plus human platelet lysate? learn more by reading our frequently asked questions - FAQS. --- [Skip to main content](https://www.compassbiomed.com/faqs#main-content) [![COMPASS New](https://www.compassbiomed.com/hs-fs/hubfs/COMPASS%20New.png?width=250&height=66&name=COMPASS%20New.png) ![COMPASS New](https://www.compassbiomed.com/hs-fs/hubfs/COMPASS%20New.png?width=250&height=66&name=COMPASS%20New.png) ![COMPASS New](https://www.compassbiomed.com/hs-fs/hubfs/COMPASS%20New.png?width=250&height=66&name=COMPASS%20New.png)](https://www.compassbiomed.com/home-page?hsLang=en) - Show submenu for Products Products - [PLUS Technology](https://www.compassbiomed.com/plus-overview) - [Research Grade](https://www.compassbiomed.com/research-grade-hpl) - [GMP Grade](https://www.compassbiomed.com/gmp-grade) - [Gamma Irradiated](https://www.compassbiomed.com/gamma-irradiated) - [Show submenu for Contract Services Contract Services](https://www.compassbiomed.com/contract-services-overview) - [Cobas Testing](https://www.compassbiomed.com/cobas-c111-analyzer-testing) - [About Us](https://www.compassbiomed.com/about-compass-biomedical) - Show submenu for Resources Resources - [Blog](https://www.compassbiomed.com/compass-biomedical-blog) - [Publications](https://www.compassbiomed.com/resource-publications) - [FAQs](https://www.compassbiomed.com/faqs) - [Distributors](https://www.compassbiomed.com/distributors) - [Contact](https://www.compassbiomed.com/contact-us) Open main navigation Close main navigation - Show submenu for Products Products - Products - [PLUS Technology](https://www.compassbiomed.com/plus-overview) - [Research Grade](https://www.compassbiomed.com/research-grade-hpl) - [GMP Grade](https://www.compassbiomed.com/gmp-grade) - [Gamma Irradiated](https://www.compassbiomed.com/gamma-irradiated) - Show submenu for Contract Services Contract Services - Contract Services - [Contract Services](https://www.compassbiomed.com/contract-services-overview) - [Cobas Testing](https://www.compassbiomed.com/cobas-c111-analyzer-testing) - [About Us](https://www.compassbiomed.com/about-compass-biomedical) - Show submenu for Resources Resources - Resources - [Blog](https://www.compassbiomed.com/compass-biomedical-blog) - [Publications](https://www.compassbiomed.com/resource-publications) - [FAQs](https://www.compassbiomed.com/faqs) - [Distributors](https://www.compassbiomed.com/distributors) - [Contact](https://www.compassbiomed.com/contact-us) - [Order Now](https://www.jotform.com/form/252014995990163) - [Order Now](https://www.jotform.com/form/252014995990163) # Frequently Asked Questions Your guide to PLUS™ Human Platelet Lysate. Answers to your most common questions. [More Questions? Get in Touch](https://www.compassbiomed.com/contact-us?hsLang=en) ### What is PLUS™ Human Platelet Lysate​ and why should I use it as a replacement for FBS? PLUS™ human platelet lysate is a cell culture supplement that supports the* in vitro* propagation and maintenance of bone marrow derived MSCs, adipose derived MSCs, cord blood derived MSCs, Fibroblasts, Keratinocytes, T-cells, HSCs, NK cells, Dendritic cells, iPSC-derived MSCs, HUC-PVCs, Tumor cells, and more. PLUS™​ human platelet lysate provides a completely xenogenic-free alternative to FBS that can significantly improve your cell culture results. Porcine derived heparin is not added during any of the manufacturing steps for PLUS™​ nor does it need to be added during cell culture for any of our projects. ### How is PLUS™ ​human platelet lysate​ different from other platelet lysate products on the market? Our proprietary manufacturing process removes coagulation factors and eliminates the need for addition of anti-coagulants, such as porcine derived heparin, to PLUS™​ human platelet lysate. PLUS™ is a true xenogenic-free finished product and does not require the addition of further heparin during cell culturing. Our products are suitable for standard culture protocols, automated processes, and bioreactors. ### Is PLUS™​ available as a GMP product? Yes. PLUS™​ human platelet lysate is available as a GMP product. Many leading institutions are testing, validating and using PLUS™​ human platelet lysate as a replacement for FBS and human AB serum in their clinical trial protocols. We anticipate a complete shift to platelet lysate for improved safety and efficacy in cellular therapy protocols based on FDA recommendations. ### How is PLUS™​ human platelet lysate manufactured? PLUS™​ human platelet lysate is manufactured from platelet apheresis units obtained from FDA-registered AABB blood banks utilizing a proprietary method that releases the platelets’ growth factors, cytokines, and other proteins, removes coagulation factors, and produces a sterile product. Our manufacturing process does not include the addition of any xenogenic additives making the final product a completely xeno-free product. Heparin is not required for cell culturing with any of our products. ### Do you use fresh or frozen platelets to manufacture PLUS? Is there any difference between fresh and frozen platelets? PLUS™​ human platelet lysate manufacturing process utilizes platelet units frozen upon expiration. Our internal quality testing and published scientific literature suggests that there is no significant difference in growth factor activity between fresh and frozen platelets. ### What is the shelf life of PLUS™​ human platelet lysate? The current recommended shelf life is 5 years at ≤-20°C. Once thawed, PLUS™​ human platelet lysate may be kept at 4°C for 4 weeks without any significant drop in performance.  ### How is PLUS™​ human platelet lysate sterilized? All PLUS™​ human platelet lysate products are sterile filtered. Each lot is tested for sterility per USP guidelines. Our Gamma Irradiated product is further sterilized via irradiation after being sterile filtered and bottled/bagged in final packaging. ### What MSCs have been tested with PLUS™​ Human Platelet Lysate? PLUS™​ human platelet lysate has undergone internal testing using human bone marrow derived (MSCs) and adipose derived stem cells (ASCs). Our customers and recent research publications have indicated that PLUS™​ human platelet lysate works well for umbilical cord derived MSCs. ### What other cell types can be grown in PLUS™​ Human Platelet Lysate? Our clients have used PLUS™​ human platelet lysate for culturing different cell types such as tumor infiltrating T-cells, endothelial cells, bone marrow derived MSCs, adipose derived MSCs, cord blood derived MSCs, Fibroblasts, Keratinocytes, HSCs, NK cells, Dendritic cells, iPSC-derived MSCs, HUC-PVCs, Tumor cells, and more with success. See our publication page for more information on current research with our product. ### What is a typical production lot size for PLUS™​ Human Platelet Lysate? Our typical lot size is 100+ liters. We can also manufacture custom sized lots, and entire lots may be reserved. ### Do you offer bags for closed loop options? Yes, we offer 100ml and 250ml bags. 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